Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion |
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Authors: | Roque P Almeida Anne Vanet Veronique Witko-Sarsat Maxine Melchior Denise McCabe Joelle E Gabay |
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Institution: | aDivision of International Medicine and Infectious Diseases, Cornell University Medical College, 1300 York Avenue, New York, New York, 10021;bDivision of Hematology—Oncology, Department of Medicine, Cornell University Medical College, 1300 York Avenue, New York, New York, 10021 |
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Abstract: | The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells. |
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