正交设计优选超声辅助提取柑桔叶精油工艺研究

采用溶剂萃取法提取柑桔叶中的精油,并优化工艺条件。通过气相色谱、质谱等仪器对所提取的精油组分进行鉴定分析。最终得到最佳提取条件:用6倍石油醚浸泡4 h,超声60次,可得到较好产率,产率1.73%。利用气相色谱-质谱联用法对柑橘叶精油的化学组成进行分析,用归一化法测定其相对百分含量,共分离出387种成分,鉴定了其中的32种成分,占出峰物质总量的70.22%,主要成分为β-氧化石竹烯(10.15%)、十六烷酸(8.15%)、芳樟醇(6.31%)、α-甜橙醛(4.33%)、亚油酸(6.48%)、视黄醛(4.04%)等成分。

Optimization of Ultrasonic Extractiing Essential Oils from Citrus reticulata Leaves by Orthogonal Design

Essential oils were extracted from Citrus reticulata leaves with ultrasonic extraction.The results showed that solvent extraction gave the best effect when 6 times solvent dipped 3 hours,60 times under ultrasonic wave,and the yield of essential oils was 1.73 %.The chemical constituents of ultrasonic extraction were determined by GC/ MS.The relative contents were determined by area normalizing method.32 constituents were identified from 387 separated peaks,amounting to total mass fraction 70.22%.The main com...