大鼠前体脂肪细胞分化过程中波形纤维形态结构的变化和波形纤维蛋白基因表达的研究

通过对不同分化阶段的大鼠前体脂肪细胞中波形纤维蛋白的结构形态和分布的间接免疫荧光观察，发现随着前体脂肪细胞的分化，波形纤维形态结构发生特异性改变，即从前期的围绕细胞核聚集且向细胞周边平行延伸到后期的围绕脂滴间隔形成致密笼状结构。此外，通过地高辛标记的寡核苷酸探针的原位杂交和免疫印迹研究了前体脂肪细胞的分化对波形纤维蛋白基因表达的影响。结果表明，分子量为57kD的波形纤维蛋白在mRNA水平和蛋白水平的表达贯穿于前体脂肪细胞分化的全过程，表达量呈递减趋势。这提示在前体脂肪细胞分化中，波形纤维与脂滴的特异性结合对于脂滴的前体脂肪细胞分化有着功能性的联系，特别是对脂肪细胞的脂滴形成极可能起到支撑的作用。

Studies of the Gene Expression and Structure Alteration of vimentin During Differentiation of Rat Preadipocytes

Observations on structure and distribution of vim entin as determined by indirect immunofluorescence were showed that the arrangement of vim entin intermediate filments (IFs) changed from an extended fibrillar state to a complex cage formation tightly associated with the forming lipid globules during adipose conversion of rat preadipocytes.The effects of differentiation of rat preadipocytes into adipocytes on vim entin mRNA levels as determined by in situ hybridization (ISH) using oligonucleotides probes to vim entin mRNA labeled by digoxigenin (DIG),and on vim entin protein expression as determined by Western blotting were researched.The results suggested that the expressions of vim entin with molecular weight of about 57 kD at the levels of both mRNA and protein was existed throughout the predipocyte differentiation and downregulation of vim entin is required for adipose conversion.The specificity of the association of lipid globules with vim entin IFs during adipose conversion was taken as an example of a possible IF function in relation to a preadipocytes differentiation process,especially discussed as a functional relationship in supporting adiposgenesis.