Effects of colchicine on DNA synthesis, endocytosis and fine structure of cultivated arterial smooth muscle cells

Confluent secondary cultures of rat arterial smooth muscle cells were exposed to cationic and anionic derivatives of ferritin and horseradish peroxidase and studied electron microscopically in order to clarify the influence of molecular net charge on surface binding and endocytosis of proteins. The cationic markers bound uniformly to the plasma membrane. They were then ingested by membrane invagination and via small vesicles transported to lysosomes and the Golgi complex. These organelles were both labelled already after 30 min of incubation. With longer exposure times (2-4 h), an increasing accumulation within the lysosomes was observed, whereas the labelling of the Golgi complex decreased. In spite of continued interiorization of plasma membrane carrying the cationic markers, the cells retained their ability to bind the latter to the surface. The anionic markers did not bind to the cell surface, were taken up in the fluid phase, and later observed only in lysosomes. If assuming that the cationic and anionic proteins serve as markers for the plasma membrane and fluid phase, respectively, but do not affect the intracellular path of interiorized membrane, these results indicate that the endocytic vesicles fuse with and empty their content into lysosomes and that part of the incoming membrane subsequently is transferred to the Golgi complex for possible recirculation back to the cell surface. If, on the other hand, the net charge of the exogenous marker influences the path of the vesicles, there may exist more than one recovery route for membrane interiorized by endocytosis.