Neocarzinostatin: spectral characterization and separation of a non-protein chromophore. |
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| Authors: | M A Napier B Holmquist D J Strydom I H Goldberg |
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| Institution: | 1. Department of Pharmacology, Harvard Medical School, Boston, Massachusetts 02115 USA;2. Biophysics Research Laboratory, Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115 USA;3. The Division of Medical Biology, Peter Bent Brigham Hospital, Boston, Massachusetts 02115 USA |
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| Abstract: | Chromatographically purified neocarzinostatin exhibits absorption, fluorescence, magnetic circular dichroic and circular dichroic spectral characteristics above and below 300 nm atypical for a protein with its reported aminoacid composition, indicating the presence of a non-protein chromophore. The drug complex, stable at acidic pH, can be dissociated by treatment with reducing or denaturing agents at neutral or basic pH. Chromatography of the dissociated complex, or more conveniently, methanol extraction of the lyophilized drug, separates a protein with an amino-acid composition identical to neocarzinostatin and a highly fluorescent chromophore free of amino-acids. |
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| Keywords: | To whom the correspondence should be addressed |
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