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Intracellular mobility and nuclear trafficking of the stress-activated kinase JNK1 are impeded by hyperosmotic stress

Authors:	Mariya Misheva Gurpreet Kaur Kevin RW Ngoei Yvonne Y Yeap Ivan HW Ng Kylie M Wagstaff Dominic CH Ng David A Jans Marie A Bogoyevitch

Institution:	1. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia;2. Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia;3. European Molecular Biology Laboratory (EMBL) Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia

Abstract:

Keywords:	MAPK mitogen-activated protein kinase ERK extracellular signal-regulated kinase JNK c-Jun N-terminal kinase Imp importin NLS nuclear localization sequence NTS nuclear translocation signal FRAP fluorescence recovery after photobleaching GFP green fluorescent protein GST glutathione S-transferase MKK MAPK kinase IAF 5-iodoacetamidofluorescein HTC cells rat hepatoma tissue culture cells CLSM confocal laser scanning microscopy TR70 Texas Red-labeled dextran MEFs murine embryonic fibroblasts p45 nuclear factor erythroid 2 (NF-E2) TAg Simian Virus SV40 large tumor antigen Fn nuclear fluorescence Fc cytoplasmic fluorescence Fn/c the nuclear to cytoplasmic ratio t_1/2 time to reach half-maximal recovery SEM standard error of the mean
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