Countercurrent chromatography--a new method for the fractionation of glycosaminoglycans |
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| Authors: | R E Hurst J Y Sheng Y Ito |
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| Institution: | 1. Department of Engineering Biophysics, University of Alabama in Birmingham, University Station, Birmingham, Alabama, 35294 USA;2. Department of Biochemistry, University of Alabama in Birmingham, University Station, Birmingham, Alabama, 35294 USA;3. the Center for Developmental and Learning Disorders, University of Alabama in Birmingham, University Station, Birmingham, Alabama, 35294 USA |
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| Abstract: | A new method is described for continuous flow fractionation of glycosaminoglycans (GAG) by partition in two-phase systems. In the 1-butanol/aqueous NaCl two-phase system containing hexadecylpyridinium chloride fractionation is dependent primarily upon chemical composition, or charge density, of the GAG and is relatively independent of molecular weight. Chondroitin 4-sulfate was fractionated according to degree of sulfation and could be completely resolved from heparin. A heparin sample was shown to contain three discrete components differing with respect to sulfaminohexose and sulfate substitution. |
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