An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose |
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| Authors: | Wang Zhanchao Chen Ming Xu Yuquan Li Shuying Lu Wei Ping Shuzhen Zhang Wei Lin Min |
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| Affiliation: | (1) College of Biological Sciences, China Agricultural University, Beijing, 100094, P.R. China;(2) Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081, P.R. China |
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| Abstract: | An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc. |
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| Keywords: | Alcohol dehydrogenase Escherichia coli Ethanol Pyruvate decarboxylase Xylose |
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