家蚕黄血抑制基因的SSR定位

家蚕黄茧性状主要由3个基因控制, 分别是黄血基因(Yellow blood, Y), 黄血抑制基因(Yellow inhibitor, I)和黄茧基因(Out-layer yellow cocoon, C)。I基因阻止类胡萝卜素从中肠上皮细胞到血淋巴的转运, 是天然黄茧形成过程中的重要控制基因。利用家蚕雌性不发生交换的特点, 采用黄血黄茧品系KY和白血白茧品系巴格达特(Ba)组配正反交群体(Ba×KY)×KY和KY×(Ba×KY), 分别记作BC1F和BC1M, 根据已经构建的家蚕SSR分子标记连锁图谱对I基因进行了定位及连锁分析。筛选出3个与I基因连锁的SSR标记。BC1F群中的所有白血个体均表现出与(Ba×KY) F1相同的杂合型带型; 而所有黄血个体带型与亲本KY一致, 为纯合型。利用另一个群体BC1M构建了关于I基因的遗传连锁图, 连锁图的遗传距离为38.4 cM, 与I基因最近的引物为S0904, 图距为7.4 cM。

Mapping of the yellow inhibitor gene I in silkworm Bombyx mori using SSR markers

The yellow color of silkworm (Bombyx mori) cocoon is mainly controlled by three genes, Y (yellow blood), I (yellow inhibitor) and C (out-layer yellow cocoon) genes. I gene locates on the 9th chromosome of silkworm and prevents the transport of carotenoid from epithelia of midgut into hemolymph. Owning to a lack of crossing over in females, reciprocal backcrossed F1(BC1) progenies were used for linkage analysis and mapping of the I gene based on the SSR linkage map using silkworm strains Baghdad (Ba), which express white hemolymph (II+Y+Y), and KY, which express yellow hemolymph (+I+IYY). The gene of interest was linked to three (S0904, S0905, and S0906) SSR markers. All the individuals with white hemolymph in the BC1F (BC1 was generated using F1 as female) showed heterozygous profile of (BaxKY) F1, and the yellow ones in BC1F showed the homozygous profile of the strain KY. Using a reciprocal BC1M cross, we con-structed a linkage map of 38.4 cM, and the distance between I gene and the nearest marker S0904 is 7.4 cM.