Spontaneous Erythromycin Resistance Mutation in a 23S rRNA Gene, rrlA, of the Extreme Thermophile Thermus thermophilus IB-21 |
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| Authors: | Steven T Gregory Jamie H D Cate and Albert E Dahlberg |
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| Institution: | J. W. Wilson Laboratory, Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA. Steven_Gregory@Brown.edu |
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| Abstract: | Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool for the genetic manipulation of the 23S rRNA genes of Thermus. |
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