非折叠蛋白质应答对人胚肾细胞293A迁移特性的影响

为了研究非折叠蛋白质应答对器官发生的影响，应用衣霉素诱导非折叠蛋白质应答并观察其对人胚肾细胞系293A迁移特性的影响。在实验中，应用划痕法对细胞迁移进行观察，并应用细胞黏附实验、荧光染色技术、扫描电镜技术及免疫印迹实验分别对细胞黏附特性、微管及微丝、细胞表面边缘的突起及小分子GTPase的表达水平进行研究。结果表明，非折叠蛋白质应答可以抑制细胞迁移，进一步的研究发现，非折叠蛋白质应答可以降低细胞的黏附能力、引起细胞骨架的重排、抑制伪足的形成并降低RhoA的表达水平。这提示，非折叠蛋白质应答可能通过抑制应激细胞的迁移为应激细胞的功能修复赢得了时间，在器官发生过程中发挥作用。

THE EFFECTS OF THE UNFOLDED PROTEIN RESPONSE(UPR)ON CELL MIGRATION IN ORGANOGENESIS IN VITRO

To investigate the influence of the unfolded protein response (UPR) on cell migration in organogenesis, we induced UPR with tunicamycin and observed the effects in vitro of UPR on the migration behaviors of human embryonic kidney cell line 293A.In the study, we examined some behaviors related to cell migration including cell adhesion, the distribution of microtubule and actin cytoskeleton, the filopodium on the leading edge and the expression level of small GTPase RhoA using scratch wound assay, cell adhesion assay, fluorescent staining, scanning electron microscopy and Western blotting respectively. The results have shown that UPR could inhibit cell migration by weakening cell adhesion to the substrate, causing the rearrangement of microtubule and actin cytoskeleton, inhibiting filopodium formation on the leading edge and decreasing the expression level of RhoA. We infer that UPR could win more time for the stressed cells for repairing their functions in the process of migration to their destination in organogenesis.