High efficiency <Emphasis Type="Italic">Agrobacterium rhizogenes</Emphasis>-mediated transformation of <Emphasis Type="Italic">Saponaria vaccaria</Emphasis> L. (Caryophyllaceae) using fluorescence selection |
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Authors: | Janice F Schmidt Maria D Moore Lawrence E Pelcher Patrick S Covello |
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Institution: | (1) Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9 Saskatoon, SK, Canada |
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Abstract: | A highly efficient and convenient method for the Agrobacterium rhizogenes-dependent production of transformed roots of Saponaria vaccaria L. (Caryophyllaceae) is described. The parameters tested and optimized include S. vaccaria cultivar, explant type, Agrobacterium rhizogenes strain and culture conditions. For cotransformation using additional recombinant T-DNA-containing A. rhizogenes strains, use of neomycin phosphotransferase and enhanced green fluorescent protein genes as selectable markers were tested
alone and in combination. Optimal results, yielding a minimum of one transformed root per explant, were obtained using the
cultivar Pink Beauty, the A. rhizogenes strain LBA9402 and internode explants precultured on a phytohormone mixture. Selection of cotransformed roots by observation
of enhanced green fluorescent protein fluorescence alone was highly effective and convenient.
NRCC Publication No. 48435. |
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Keywords: | Saponaria vaccaria Caryophyllaceae Agrobacterium rhizogenes Transformation Green fluorescent protein (GFP) |
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