Distribution of loosely-bound calcium in the vegetative and generative cells of the pollen grains in Chlorophytum elatum |
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Authors: | A. Górska-Brylass R. Butowt M.I. Rodriguez-Garcia |
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Affiliation: | (1) Institute of Biology, Copernicus University, PL-87100 Torun, Poland;(2) Institute for Plant Breeding and Acclimatization, PL-85950 Bydgoszcz, Poland;(3) Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estacion Experimental del Zaidin (C.S.I.C.), E-18008 Granada, Spain |
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Abstract: | We used chlorotetracycline fluorescence, alizarin staining and potassium pyroantimonate methods, as well as X-ray microanalysis, to demonstrate the differential localization of Ca2+ during pollen maturation. Level of loosely-bound Ca2+ ions was higher in the generative cell than in the vegetative cell of the mature pollen grain which is one of the symptoms of metabolic differentiation of the two sister cells. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | ATPase chlorotetracycline cytochemistry pollen grain X-ray microanalysis |
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