Cross-reactivity of metallothioneins from different origins with rabbit anti-rat hepatic metallothione in antibody. |
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| Authors: | C Tohyama Z A Shaikh |
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| Institution: | Department of Pharmacology and Toxicology, University of Rochester School of Medicine and Dentistry Rochester, New York 14642 USA |
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| Abstract: | Rat hepatic Cd-metallothionein was purified and isolated into its two components, metallothionein 1 and 2, by disc electrophoresis. Antibodies to metallothionein 2 were generated in rabbits. The antiserum reacted with the protein and formed a single precipitin band on a double diffusion plate. By ammonium sulfate precipitations, it was found that the antiserum cross-reacted with rat hepatic metallothionein 1. Cross-reactivity of the antiserum was also observed for components of rat renal Cd-metallothionein, rabbit hepatic Cd-metallothionein and human renal metallothionein. |
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| Keywords: | MT(s) metallothionein(s) FCA Freund's complete adjuvant FIA Freund's incomplete adjuvant |
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