A One-Bead One-Peptide Combinatorial Library Method for B-Cell Epitope Mapping |
| |
Authors: | Kit S. Lam Douglas Lake Sydney E. Salmon John Smith Man-Ling Chen Shelly Wade Farid Abdul-Latif Richard J. Knapp Zuzana Leblova Ronald D. Ferguson Viktor Krchnak Nikolai F. Sepetov Michal Lebl |
| |
Affiliation: | aArizona Cancer Center and Department of Medicine, University of Arizona College of Medicine, Tucson, Arizona, 85724;bDepartment of Microbiology and Immunology, University of Arizona College of Medicine, Tucson, Arizona, 85724;cSelectide Corporation, 1580 E. Hanley Boulevard, Tucson, Arizona, 85737 |
| |
Abstract: | The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide–beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-β-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) anti-human insulin (discontinuous epitope), and (iv) surface immunoglobulins (μκ) of two murine B-cell lymphoma cell lines (antigen unknown). |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|