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Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12 |
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| Authors: | M G Marinus A Poteete J A Arraj |
| Affiliation: | 1. Department of Pharmacology University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01605 U.S.A. Tel. (617) 856-3330 |
| Abstract: | Using a multicopy plasmid in which the tac promoter has been placed in front of the dam gene of Escherichia coli K-12, we show that levels of DNA adenine methylase activity are correlated with the spontaneous mutation frequency. |
| Keywords: | Recombinant DNA multicopy plasmid mismatch repair ß-lactamase gene bp base pairs gene encoding an enzyme which methylates-GATC-sequences in DNA (Marinus, 1984) IPTG isopropylthio-ß-galactopyranoside 6-meA 6-methyladenine naladixic acid resistance p plasmid rifampicin resistance streptomycin resistance |
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