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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries |
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| Authors: | Sheila Fisher Andrew Barry Justin Abreu Brian Minie Jillian Nolan Toni M Delorey Geneva Young Timothy J Fennell Alexander Allen Lauren Ambrogio Aaron M Berlin Brendan Blumenstiel Kristian Cibulskis Dennis Friedrich Ryan Johnson Frank Juhn Brian Reilly Ramy Shammas John Stalker Sean M Sykes Jon Thompson John Walsh Andrew Zimmer Zac Zwirko Stacey Gabriel Robert Nicol Chad Nusbaum |
| Institution: | 1Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA;2Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA;3Genetic Analysis Platform, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA;4Foundation Medicine, One Kendall Square, Suite B6501, Cambridge, MA 02139, USA |
| Abstract: | Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol. |
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