Inositol(3,4)bisphosphate and inositol(1,3)bisphosphate in GH4 cells--evidence for complex breakdown of inositol(1,3,4)trisphosphate |
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| Authors: | R F Irvine A J Letcher D J Lander J P Heslop M J Berridge |
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| Institution: | 1. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, U.K.;2. AFRC Unit of Invertebrate Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, U.K.;1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125 Berlin, Germany;2. Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany;1. Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, Germany;2. Department of Chemistry and Pharmacy and CIBSS-Centre for Integrative Biological Signalling Studies, Albert-Ludwigs University Freiburg, 79104 Freiburg, Germany;3. Medical Research Council Laboratory for Molecular Cell Biology (MRC-LMCB), University College London, London WC1E 6BT, UK;4. Department of Biochemistry, Indian Institute of Science, Bengaluru, Karnataka 560 012, India;5. Leibniz-Forschungsinstitut für Molekulare Pharmakologie, 13125 Berlin, Germany;6. Department of Chemistry, Humboldt Universität zu Berlin, 12489 Berlin, Germany;7. Institute of Agronomy and Crop Physiology, Justus-Liebig University Giessen, 35392 Giessen, Germany;8. Department of Physiology & Cell Biology, Leibniz-Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany;1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125 Berlin, Germany;2. Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany |
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| Abstract: | Analysis of inositol bisphosphates in GH4 cells labelled with 3H]myo-inositol shows that these cells contain three detectable inositol bisphosphates: inositol(1,4)bisphosphate, and two novel inositol bisphosphates. These latter inositol bisphosphates were degraded by periodate oxidation, borohydride reduction and alkaline phosphatase dephosphorylation; each yielded single non-cyclic alditols, ribitol and threitol, indicating that they must be respectively inositol(1,3)bisphosphate and inositol(3,4) bisphosphate. These two inositol bisphosphates are putative breakdown products of inositol(1,3,4)trisphosphate, and their occurrence suggests a complex route of hydrolysis of inositol(1,3,4)trisphosphate in intact cells. |
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