Effect of Chlorhexidine on Multi-Species Biofilms |
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Authors: | Michael Wilson Haziq Patel Joseph H Noar |
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Institution: | (1) Department of Microbiology, Eastman Dental Institute, University of London, 256 Grays Inn Road, London WC1X 8LD, UK , GB;(2) Department of Orthodontics, Eastman Dental Institute, University of London, 256 Grays Inn Road, London WC1X 8LD, UK , GB |
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Abstract: | With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor
(CDFF) in the presence (suc +ve) and absence (suc −ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate
(CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve biofilms had higher proportions of streptococci but lower
proportions of veillonellae than suc −ve biofilms. Exposure to CHG for 1 min reduced the viable count of suc −ve and suc +ve
biofilms by 53% and 89% respectively. In both cases, reductions were mainly attributable to killing of streptococci and actinomyces.
After 60 min of exposure, 4% of the bacteria in the suc −ve and 2% in the suc +ve biofilms remained viable. This study has
shown that large numbers of bacteria in microcosm dental plaques can survive a 1-min exposure to 0.2% CHG and that even after
a 60-min exposure, substantial numbers of bacteria remain viable.
Received: 16 May 1997 / Accepted: 7 July 1997 |
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