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Genetic diversity of Triticum turgidum L. based on microsatellite markers
Authors:W. Li  D. -F. Zhang  Y. -M. Wei  Z. -H. Yan  Y. -L. Zheng
Affiliation:(1) Ministry of Education Key Laboratory of Southwest Crop Genetic Resources and Improvement, Sichuan Agricultural University, Yaan, Sichuan, 625014, China;(2) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830, China;(3) College of Agronomy, Sichuan Agricultural University, Yaan, Sichuan, 625014, China
Abstract:Using microsatellite (SSR) markers, the genetic diversity and genetic relationships among 48 Triticum turgidum L. accessions, including 30 Triticum turgidum L. ssp. turgidum, 7 Triticum turgidum L. ssp. durum, 4 Triticum turgidum L. ssp. carthlicum, 3 Triticum turgidum L. ssp. paleocolchicum, 2 Triticum turgidum L. ssp. turanicum, and 2 Triticum turgidum L. ssp. polonicum accessions, were investigated. A total of 97 alleles were detected at 16 SSR loci. At each locus, the number of alleles ranged from two to fourteen, with an average of 6.1. The genetic similarity (GS) value ranged from 0.20 to 0.92, with the mean of 0.59. In cluster analysis, it was found the 48 Triticum turgidum L. accessions could be distinguished easily by SSR markers, whereas the six subspecies taxonomic entities of T. turgidum L. could not differentiate with each other, indicating that the morphological differences present among the six subspecies could not be reflected by the SSR markers. These results suggested that SSR markers had superiority in detecting the genetic diversity of T. turgidum L., while they were not good for studies of the phylogenic relationships among the subspecies of T. turgidum L. The text was submitted by the authors in English.
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