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Enzyme activity measurements on isolated organs of Brachionus plicatilis (Rotifera)
Authors:Kleinow  W  Röhrig  A
Institution:(1) Zoologisches Institut der Universität zu Köln, Weyertal 119, D-50923 Köln, Germany
Abstract:A method is described by which the integument of Brachionus plicatilis, together with its intracellular lamina, is quickly dissolved before other parts or tissues of the animal are destroyed. After removing the integument several parts of the body can be separated and fractionated in a more or less intact state by centrifugation in a Percoll gradient. The measurement of enzyme activities has indicated that this procedure might provide a way of localizing enzymes within the rotifer body.
Keywords:Enzyme localization  agr-amylase" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-amylase  beta-glucosidase" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-glucosidase  beta-galactosidase" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-galactosidase  lorica structure
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