| 血管平滑肌细胞的体外氧化应激反应及雌激素的影响 |
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| 引用本文： | 徐菁,朱从丽,王乔,宋健. 血管平滑肌细胞的体外氧化应激反应及雌激素的影响[J]. 中国组织化学与细胞化学杂志, 2010, 19(6): 537-541. DOI: 10.3870/zgzzhx.2010.06.003 |
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| 作者姓名： | 徐菁 朱从丽 王乔 宋健 |
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| 作者单位： | 1. 南华大学医学院人体解剖学教研室,湖南,衡阳,421001
2. 武汉大学医学院人体解剖学与组织胚胎学系、医学研究中心,湖北,武汉,430071 |
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| 基金项目： | 国家自然科学基金(30470906); 中澳科技合作特别基金(30711120185) |
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| 摘 要： | 目的探讨雌激素对VSMC氧化应激反应的影响及机制。方法第3-4代雌性大鼠血管平滑肌细胞(VSMC)在有或无10-8mol/L 17b-雌二醇(E2)存在下用不同浓度(0-250mmol/L)H2O2诱导氧化应激;MTT法,流式细胞术,Western blot分别检测VSMC活力,细胞周期,MAPK信号通路活性的变化。结果当浓度低于25mmol/L时,H2O2对VSMC活力无影响;当浓度为50-150mmol/L时,VSMC活力呈剂量依赖性下降,并伴随G0/G1期细胞升高;当浓度达到200mmol/L时,细胞活力下降至最低点并出现凋亡峰。10-8mol/L E2可显著抑制150mmol/L H2O2诱导的VSMCERK和p38的磷酸化、从而缓解VSMC G0/G1期阻滞,并降低高浓度H2O2诱导的VSMC凋亡。结论中等浓度的H2O2(50-150mmol/L)抑制VSMC增殖;高浓度H2O2(≥200mmol/L)不仅抑制VSMC生长、且导致部分VSMC凋亡;雌激素可通过抑制ERK和p38活性对氧化应激的VSMC起保护作用。
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| 关 键 词： | 雌激素 血管平滑肌细胞 氧化应激 过氧化氢 |
RESPONSES OF VASCULAR SMOOTH MUSCLE CELLS TO OXIDATIVE STRESS IN VITRO AND THE EFFECTS OF ESTROGEN |
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| Xu Jing,Zhu Congli,Wang Qiao,Song Jian. RESPONSES OF VASCULAR SMOOTH MUSCLE CELLS TO OXIDATIVE STRESS IN VITRO AND THE EFFECTS OF ESTROGEN[J]. Chinese Journal of Histochemistry and Cytochemistry, 2010, 19(6): 537-541. DOI: 10.3870/zgzzhx.2010.06.003 |
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| Authors: | Xu Jing Zhu Congli Wang Qiao Song Jian |
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| Affiliation: | Xu Jing1,Zhu Congli2,Wang Qiao2,Song Jian2(1Department of Anatomy,Medical Collage,University of South China,Hunan,Hengyang 421001,2Department of Anatomy,Histology and Embryology,Medical Research Center,Wuhan University School of Medicine,Wuhan 430071,China) |
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| Abstract: | Objective To investigate the effects of estrogen on the responses of vascular smooth muscle cells(VSMCs) to oxidative stress.Methods The sub-cultured(passage 3-4) female rat VSMCs were treated with different concentrations(10-250mol/L) of H2O2 in the presence or absence of 10-8mol/L E2.The viability,cell cycle progress and MAPK signaling activity were analysed with MTT assay,flow cytometry and Western blot,respectively.Results When the VSMCs were treated with 10-25mol/L H2O2,the cellular viability had no si... |
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| Keywords: | Estrogen Vascular smooth muscle cells Oxidative stress H2O2 |
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