人免疫缺损病毒蛋白印迹法的改进

IMPROVEMENT OF WESTERN BLOT TEST FOR DETECTION OF ANTIBODY TO HUMAN IMMUNODEFICIENCY VIRUS ( HIV )

To overcome the disadvantages of the tranditional Western blot ( WB ) , such as complicated procedures,sophisticaed instrument and expensive reagents used in HIV antigen purification and aptibody detection, a series of improvements were made. The lysate of HlV infected cells was directly electrophoresed and transferred to nitrocellulose paper. The complicated Cohen buffer was substituted by PBS buffer containing calf serum and the total incubation time in antibody detection can be reduced to one hour. The results showed that all major HIV proteinbends can be clearly seen with a very low nonspecific background. It was proved that the improved WB is a hig hly effective, accurate and simple method which can be applied to the antigen identification and antibody detection for HIV,and possibly for other viruses.