电针对脑缺血再灌注大鼠海马神经元GluR-2表达的影响

目的探讨电针对短暂性局灶性脑缺血再灌注损伤区神经元谷氨酸受体-2(GluR-2)表达的影响及其意义。方法手术制作中动脉栓塞(MCAO)大鼠模型;应用免疫组织化学方法检测海马CA1区GluR-2的定位表达,并应用免疫印迹(Western blot)方法对其进行定量分析。结果免疫组化结果:正常组、假手术组大鼠海马CA1区GluR-2的阳性反应强度无明显差别(P>0.05);MCAO模型组大鼠海马CA1区GluR-2的阳性反应比正常组和假手术组明显减弱(P<0.05);电针 MCAO模型组海马CA1区GluR-2的阳性反应比手术组明显增强,但比正常组和假手术组仍显偏弱(P<0.05)。Western blot结果与免疫组化结果一致。结论本试验的结果提示电针针刺可上调GluR-2的表达,从而对神经元起到一定保护作用。

EFFECT OF ELECTROACUPUNCTURE ON GLUR-2 EXPRESSION OF RAT HIPPOCAMPAL NEURONS IN BRAIN ISCHEMIA

Objective To investigate the effect of electro-acupuncture on GluR-2 expression after brain ischemia.Methods The expression of GluR-2 in the neurons of the CA1 region of rat hippocampus was detected by immunohistochemical method and Western blot.Computer-assisted image analysis was used for quantitative analysis.Results Immunohistochemically,the immunoreaction of GluR-2 positive neurons was not obviously different between the sham and the control groups(P>0.05),but was decreased in the ischemia group(P<0.05).The immunoreaction of GluR-2 positive neurons was increased in acupuncture group,but still weaker than that of the control and sham group(P<0.05).Results of Western blot were coincident with those of immunohistochemistry.Conclusion Electro-acupuncture can up-regulate the expression of GluR-2 in cerebral ischemia,which might play an important role in protecting the neurons of rat hippocampus from the damage induced by ischemia.