The effect of thiols onSaccharomyces fragilis

Treatment of cell suspensions ofSaccharomyces fragilis with 0.01m β-mercaptoethanol or dithiothreitol released a variety of substances of high and low molecular weight. Twenty-two high-molecular-weight glycoproteins were separated by a combination of chromatography on DEAE cellulose and polyacrylamide gel electrophoresis in presence of sodium dodecylsulphate. The carbohydrate components consisted of at least 95% mannose and the protein components had threonine and serine as the major amino acids. Only very small amounts of phosphorus were associated with the high-molecular-weight components. The low-molecular-weight substances were probably released from the internal cell pool and uracil and hypoxanthine were identified as components of this fraction. It is suggested that in addition to breaking disulphide bridges in the cell wall the thiols may also render the plasmalemma permeable to certain low-molecular-weight substances. Such effects are not lethal since the yeast can be trained to grow in presence of 0.01m mercaptoethanol.