| 银离子对辣根过氧化物酶的影响 |
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| 引用本文： | 刘成,韩宏岩,张颖,许维岸.银离子对辣根过氧化物酶的影响[J].生物技术,2006,16(4):49-52. |
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| 作者姓名： | 刘成 韩宏岩 张颖 许维岸 |
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| 作者单位： | 1. 山东农业大学生命科学学院,江苏,泰安,271018
2. 苏州大学生命科学学院,江苏,苏州,215006 |
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| 摘 要： | 实验研究Ag 对HRP的影响对检测银的污染有重要意义。以ABTS2,2-连氮-双-(3-乙基苯并噻唑-6-磺酸)]和H2O2为底物,在pH值5.0的条件下,用分光光度法考察了Ag 存在下的辣根过氧化物酶催化氧化反应。Ag 对辣根过氧化物酶的催化活性显示出抑制作用,并进一步分别探讨了对两种底物的抑制类型和对酶结构的影响。结果表明Ag 对底物H2O2而言,对酶的抑制效应属于反竞争性抑制类型,抑制常数Ki=14.83mmol/L;对底物ABTS而言,对酶的抑制效应属于非竞争性抑制,抑制常数Ki=16.139mmol/L。不同浓度Ag 分别与酶作用后,测定酶的内源荧光光谱。光谱结果表明Ag 影响酶活性的同时也影响酶的构象。
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| 关 键 词： | 辣根过氧化物酶 银离子 抑制 |
| 文章编号： | 1004-311X（2006）04-0049-04 |
| 收稿时间： | 2005-10-28 |
| 修稿时间： | 2006-02-16 |
Effects of Ag+ on the Catalytic Oxidation by HRP |
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| LIU Cheng,HAN Hong-yan,ZHANG Ying,XU Wei-an.Effects of Ag+ on the Catalytic Oxidation by HRP[J].Biotechnology,2006,16(4):49-52. |
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| Authors: | LIU Cheng HAN Hong-yan ZHANG Ying XU Wei-an |
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| Institution: | 1 .Shandong Agriculture University,Taian 271018;2.Suzhong University, Suzhou 215006,China |
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| Abstract: | Studying the effects of Ag~ on HRP has the important meaning to test silver pollution.The effects of Ag~ on the oxidation of ABTS2,2'-azino-di-(3-ethylbenziazobine sulfonate-6)] catalyzed by HRP were studied spectrophotometryly under condition of pH 5.0.The results show that Ag~ has obvious inhibition on the HRP activity.As far as H_2O_2,the nature of inhibition is uncompetitive type,Ki=14.83mmol/L.As far as ABTS,the nature of inhibition is noncompetitive type,Ki=16.139mmol/L.Surveying fluorescence,Ag~ influenced the conformation of HRP. |
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| Keywords: | horseradish peroxidase Silver ion inhibition |
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