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In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.) |
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| Authors: | Hiroaki Kiyohara Chikara IchinoYuka Kawamura Takayuki Nagai Noriko SatoHaruki Yamada Maha M SalamaEssam Abdel-Sattar |
| Institution: | a Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan b Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan c Oriental Medicine Research Center, Kitasato University, Tokyo, Japan d School of Pharmacy, Kitasato University, Tokyo, Japan e Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt f Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia |
| Abstract: | Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10 μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-d-glucosyl (1 → 4)-β-d-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1 μg/ml (IC50 = 0.86 μg/ml). |
| Keywords: | Adenium obesum Tephorosia nubica Oleandrogenin-glucosyldigitaloside Antiviral Influenza virus A/PR/8/34 (H1N1) MDCK cells MTT assay |
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