Characterization of a birch (Betula pendula Roth.) embryogenic gene,BP8 |
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| Authors: | Riitta Puupponen-Pimiä Markku Saloheimo Tuija Vasara Runar Ra Janka Gaugecz Ulrika Kurtén Jonathan K. C. Knowles Sirkka Keränen Veli Kauppinen |
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| Affiliation: | (1) VTT Biotechnical Laboratory, PO BOX 202, SF-02151 Espoo, Finland;(2) Present address: Department of Plant Biology, University of Helsinki, SF-00710 Helsinki, Finland;(3) Present address: Department of Genetics, University of Helsinki, SF-00100 Helsinki, Finland;(4) Present address: Department of Biochemistry and Food Technology, Technical University of Budapest, P.O. Box 91, H-1521 Budapest, Hungary;(5) Present address: Glaxo Institute for Molecular Biology S.A., 14, Chemin des Aulx, 1228 Plan-les Quates, Geneva, Switzerland |
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| Abstract: | We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene. |
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| Keywords: | Betula pendula Roth. birch embryogenic genes somatic embryogenesis |
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