Cloning and analysis of a genomic fragment of Dictyostelium discoideum hybridizing to an RNA specifically accumulated in spore cells |
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Authors: | Julien J Camonis J Jacquet M |
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Institution: | Institut de Biochimie, Batiment 432, Université de Paris-Sud, 91405 Orsay Cédex, France. |
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Abstract: | A marker for Dictyostelium discoideum spore RNA differentiation has been isolated from a genomic DNA library by differential screening and a recombinant plasmid containing a genomic sequence complementary to spore specific RNA has been characterized. Only a small portion (~1 kb) of the 4.7-kb genomic insert is transcribed. The genomic organization of this spore specific gene shows a unique sequence flanked by reiterated sequences. |
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