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A Physical Map of Arabidopsis thaliana Chromosome 3 Represented by Two Contigs of CIC YAC, P1, TAC and BAC Clones |
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| Authors: | Sato, Shusei Kotani, Hirokazu Hayashi, Reiko Liu, Yao-Guang Shibata, Daisuke Tabata, Satoshi |
| Affiliation: | 1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan 2Chiba Prefectural Agricultural Experiment Station 2-325 Sawara, Sawara, Chiba 287-0822, Japan 3Mitusi Plant Biotechnology Research Institute TCI D-21, 2-1-6 Sengen, Tsukuba, Ibaraki 305-0047, Japan 4Genetic Engineering Laboratory, South China Agricultural University Guangzhou 510642, People's Republic of China |
| Abstract: | We have constructed a physical map of Arabidopsis thaliana chromosome3 by ordering the clones from CIC YAC, P1, TAC and BAC librariesusing the sequences of a variety of genetic and EST markersand terminal sequences of clones. The markers used were 112DNA markers, 145 YAC end sequences, and 156 end sequences ofP1, TAC and BAC clones. The entire genome of chromosome 3, exceptfor the centromeric and telomeric regions, was covered by twolarge contigs, 13.6 Mb and 9.2 Mb long. This physical map willfacilitate map-based cloning experiments as well as genome sequencingof chromosome 3. The map and end sequence information are availableon the KAOS (Kazusa Arabidopsis data Opening Site) web siteat http://www.kazusa.or.jp/arabi/. |
| Keywords: | Arabidopsis thaliana chromosome 3 physical map contig map |
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