Expression and immunoreactivity of a human group a rotavirus Vp4 |
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Authors: | Qing-huan Zhao Yu-ling Wen Yang Yu Qing Dai Yuan-ding Chen |
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Affiliation: | Institute of Medical Biology of Chinese Academy Medical Sciences &Peking Union Medical College, Kunming 650118, China |
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Abstract: | Rotavirus capsid protein Vp4 plays an important role in the virus adhering and entering the cells. In this study, a Vp4 gene cloned from a rotavirus strain TB-Chen was highly expressed in E. coli BL21 (DE3). The results of the Western blot showed that the protein possesses specific immuno-reactivities and can be specifically recognized by guinea pig antibodies against rotavirus strain SA11 or Wa. Some Vp4 dimers were formed during renaturation. These data obtained from this study provide a strong basis for further study on the structure and function of the Vp4. |
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Keywords: | Recombinant rotavirus VP4 Prokaryotic expression Immunoreactivity |
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