Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1 |
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Authors: | Wenyen Wang and Jennifer A Thomson |
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Institution: | (1) Department of Microbiology, University of Cape Town, 7700 Rondebosch, South Africa |
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Abstract: | Summary The nucleotide sequence of a 3.6 kb DNA fragment containing a cellodextrinase gene (celA) fromRuminococcus flavefaciens FD-1 was determined. The gene was expressed from its own regulatory region inEscherichia coli and a putative consensus promoter sequence was identified upstream of a ribosome binding site and a TTG start codon. The complete amino acid sequence of the CeIA enzyme (352 residues) was deduced and showed no significant homology to cellulases from other oganisms. Two lysozymetype active sites were found in the amino-terminal third of the enzyme. InE. coli the cloned CeIA protein was translocated into the periplasm. The lack of a typical signal sequence, and the results of transposonphoA mutagenesis experiments indicated that CeIA is secreted by a mechanism other than a leader peptide.Abbreviations CMCase
carboxymethylcellulase
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celA
gene coding for CeIA
- CelA
cellodextrinase
- ORF
open reading frame
- phoA
gene encoding alkaline phosphatase
- pNPC
p-nitrophenyl- -d-cellobioside |
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Keywords: | Ruminococcus flavefaciens Cellodextrinase Sequence Secretion |
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