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Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and sphingopyxis-like strain S32
Authors:Aranda Carlos  Godoy Félix  Becerra José  Barra Ricardo  Martínez Miguel
Institution:(1) Laboratorio de Biorremediación, Departamento de Microbiología, Facultad de Ciencias Biológicas, Chile;(2) Laboratorio de Fitoquímica, Facultad de Ciencias Naturales y Oceanográficas, Chile;(3) Unidad de Sistemas Acuáticos, Centro EULA-Chile, Universidad de Concepción, Chile
Abstract:This paper reports 2,4,6-trichlorophenol (246TCP) degradation bySphingopyxis chilensis S37 and Sphingopyxis chilensis-like strain S32,which were unable to use 246TCP as the sole carbon and energy source. In R2A broth, the strainsdegraded 246TCP up to 0.5 mM. Results with mixtures of different 246TCP and glucose concentrations in mineral salt media demonstrated dependence on glucose to allow bacterial growth and degradation of 246TCP. Strain S32 degraded halophenol up to 0.2 mM when 5.33 mM glucose was simultaneously added, while strain S37 degraded the compound up to 0.1 mM when 1.33 mM glucose was added. These 246TCP concentrations were lethal for inocula in absence of glucose. Stoichiometricreleases of chloride and analysis by HPLC, GC-ECD and GC-MS indicated 246TCP mineralisation by both strains. To our knowledge, this is the first report of bacteriaable to mineralize a chlorophenol as a non-growth and inhibitory substrate. The concept of secondary utilization instead of cometabolism is proposed for this activity.
Keywords:biodegradation  chlorophenol  cometabolism  non-growth substrate  secondary utilization  toxicity
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