Production of aroma compounds from strawberry cell suspension cultures by addition of precursors |
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Authors: | Yeong-Ching Hong Li-Chung Huang Gary A. Reineccius Susan K. Harlander Theodore P. Labuza |
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Affiliation: | (1) Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, 55108 St. Paul, MN, USA |
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Abstract: | The potential of using short-chain fatty acids and -keto-acid as precursors for the production of typical fruit-type aroma compounds by strawberry cell suspension cultures was investigated. Analysis of the headspace by gas chromatography revealed that supplemented strawberry cell suspension cultures were capable of producing low concentrations of ethyl butyrate and butyl butyrate, and converting -ketovalerate to butanal and butanol. No aroma compounds were produced in unsupplemented or heat-treated cell suspension cultures. The results indicated that esterase, decarboxylase, and alcohol dehydrogenase might exist in strawberry cell cultures. Increasing temperature, illumination and addition of mannitol favoured the production of butyl butyrate. No difference was found between one- and two-week-old cultures in the ability to convert precursors to corresponding aroma compounds. |
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Keywords: | aroma flavour precursor secondary metabolite suspension culture strawberry |
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