首页 | 本学科首页   官方微博 | 高级检索  
     


Molecular genetic analysis of membrane protein topology
Authors:C Manoil  D Boyd  J Beckwith
Affiliation:1. C. Manoil is in the Department of Genetics, University of Washington, Seattle, WA 98195, USA;2. D. Boyd and J. Beckwith are in the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA;1. Department of Ophthalmology, Capital and Coast DHB, Wellington, New Zealand;2. Royal Victoria Eye and Ear Hospital, Melbourne, Australia;3. Capital Eye Specialists, Wellington, New Zealand;1. Department of Biology, Shahed University, Tehran, Iran;2. Molecular Microbiology Research Center and Department of Biology, Shahed University, Tehran, Iran;3. Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran;1. College of Science, Engineering and Environment, University of Newcastle, Callaghan, NSW, Australia;2. ARC Centre of Excellence in Synthetic Biology, Macquarie University, North Ryde, NSW, Australia;3. Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia;4. College of Science and Engineering, Flinders University, Bedford Park, SA, Australia;5. School of Natural Sciences, Macquarie University, North Ryde, NSW, Australia;6. Hunter Biological Solutions, Newcastle, NSW, Australia;1. Department of Chemistry and Biosciences, School of Chemical and Biotechnology, SASTRA University, Kumbakonam, 612001, Tamilnadu, India;2. Actinomycetes Bioprospecting Lab Centre for Research in Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudram, Thanjavur, 613401, Tamilnadu, India
Abstract:A transmembrane protein contains domains residing in the aqueous compartments on both sides of the membrane in which it is integrated. A determination of the topology of such a protein requires the definition of which domains lie on which side of the membrane. In E. coli, mutants and gene fusions have been used to obtain this topological information.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号