| 矿物作用下A.ferrooxidans中磁小体相关基因的差异表达 |
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| 引用本文： | 刘新星,张剑,郭玉洁,郭宁.矿物作用下A.ferrooxidans中磁小体相关基因的差异表达[J].生物技术,2010,20(1):21-24. |
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| 作者姓名： | 刘新星 张剑 郭玉洁 郭宁 |
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| 作者单位： | 中南大学资源加工与生物工程学院,湖南,长沙,410083 |
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| 基金项目： | 国家自然科学基金项目，创新研究群体科学基金项目 |
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| 摘 要： | 目的:研究氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,A.f)中与磁小体形成相关的mpsA、magA、thy和mamB基因分别在黄铁矿、黄铜矿、磁黄铁矿和闪锌矿的作用下的表达差异,寻找有利于磁小体形成的最佳培养矿物能源。方法:测量以不同硫化矿为能源时的菌体生长特性,用实时定量PCR方法研究与磁小体形成相关基因的表达差异。结果:在以磁黄铁矿为能源时,菌的生长量及多数基因的表达量优于其它三种矿,四个基因相对表达量分别为1.15、2.35、1.32、2.68。结论:磁黄铁矿是A.f中磁小体形成的最佳矿物能源。
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| 关 键 词： | 氧化弧铁硫杆菌 磁小体 硫化矿 实时定量PCR |
Expression of Magnetosome Formation Genes in A.ferrooxidans under Different Minerals |
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| LIU Xin-xing,ZHANG Jian,GUO Yu-jie,GUO Ning.Expression of Magnetosome Formation Genes in A.ferrooxidans under Different Minerals[J].Biotechnology,2010,20(1):21-24. |
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| Authors: | LIU Xin-xing ZHANG Jian GUO Yu-jie GUO Ning |
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| Institution: | LIU Xin-xing,ZHANG Jian,GUO Yu-jie,GUO Ning(Department of Bioengineering,School of Resources Processing , Bioengineering,Central South University,Changsha 410083,China) |
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| Abstract: | Objective: To analyze the response of mpsA,magA,thy and mamB genes of A.ferrooxidans under different minerals including pyrite,chalcopyrite,pyrrhotite and sphalerite,find the best energy mineral for the formation of magnetosome.Method: The physiological characterization of A.ferrooxidans was measured and the expression of magnetosome formation genes under different sulfide minerals was tested by using Real-time PCR analysis.Result: Under pyrrhotite as energy source,both the quantity of cells and the express... |
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| Keywords: | Acidithiobacillus ferrooxidans magnetosome sulfide minerals Real-time PCR |
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