Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye |
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Authors: | J B Hunter S M Hunter |
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Affiliation: | MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories, Carshalton, Surrey, United Kingdom. |
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Abstract: | A simple assay for proteins in the low nanogram range is described. Proteins are bound to nitrocellulose paper in a band, stained with the commercially available colloidal gold stain AuroDye (a registered trademark of Jansen Life Sciences Products), and quantified by scanning densitometry: 1.25 to 20 ng of protein can be consistently measured. Variation in the staining intensity of different proteins is discussed. DNA, RNA, polyvinylpyrrolidone, Ficoll, and 6 M urea do not affect the assays, and samples from cellular material in a simple lysis buffer containing 0.1% of sodium dodecyl sulfate can be assayed. |
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