RNA synthesis in Escherichia coli during K + -depletion |
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Authors: | M I Bloomstein H L Ennis |
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Institution: | Roche Institute of Molecular Biology Nutley, New Jersey 07110 USA |
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Abstract: | During K+ depletion of a mutant of which cannot concentrate this cation, protein synthesis is inhibited but RNA formation continues. The RNA produced during K+ depletion was analyzed by gel electrophoresis. It was found that 4S, 5S and 23S RNA were synthesized by K+-depleted cells whether uninfected or infected with phage T4. In addition, an RNA species moving close to 16S (presumably 17S) and material of about 6–10S were made during K+ depletion. These species of RNA were not evident in growing cells. Methylation of RNA is severely inhibited during K+ depletion. |
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