| 粟酒裂殖酵母钙调素基因在大肠杆菌中的表达及表达产物的分离纯化 |
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| 引用本文： | 尹丽红,袁生. 粟酒裂殖酵母钙调素基因在大肠杆菌中的表达及表达产物的分离纯化[J]. 微生物学杂志, 1998, 18(1): 10-13 |
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| 作者姓名： | 尹丽红 袁生 |
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| 作者单位： | 南京师范大学生物系!210097 |
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| 摘 要： | 用带有粟桌酒裂殖酵母钙调索基因的质粒PEC/PCA和质粒PSB6来转化大肠杆菌SB1，扩增培养后破碎离心，上清液经Phenyl-SepharoseCL－4B柱、DEAEcellulose-52柱、SephedesG－75柱分离，得到纯化的表达产物，此产物经SDS—PAGE电泳鉴定和对环核苷酸：酷酶（cyclicnucleotidephosphodiesterase，PDE）活性的激活鉴定，确定为酵母钙调素，且其分产量小于猪脑钙调素。
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| 关 键 词： | 粟酒裂殖酵母 钙调素基因 表达产物 分离纯化 |
EXPRESSION OF CALMODULIN GEIE OF SCHIZOSACCHAROMYCES POMBE IN ESCHERICHIA COLI AND PURIFICATION AND ISOLATION OF ITS EXPRESSION RPODUCT |
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| Yin Lihong et al. EXPRESSION OF CALMODULIN GEIE OF SCHIZOSACCHAROMYCES POMBE IN ESCHERICHIA COLI AND PURIFICATION AND ISOLATION OF ITS EXPRESSION RPODUCT[J]. Journal of Microbiology, 1998, 18(1): 10-13 |
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| Authors: | Yin Lihong et al |
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| Abstract: | The plasmid PEC/PCAM carrying CaM gene of S. Pomhe and the PLaswid pSB6 carrying ly-sis gene were transfered into E. coli stain SB1. SB1 was amplified , then lysed and cen-trifuged. The smprtanent was brought to Phenyl - Sephaare CL - 4B, DEAE Cellulose - 52,SePhedex G- 75 coumn separately, the purified product was obtained. This product wa as-sayed by SDS - PAG electrophoresis and by Ca2 - dependent Phos phediesterase, it wasverified that the product was CaM, and its moecular weight is lower than Porcine brainCaM. |
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| Keywords: | Schizosaccharomyces pombe CaM gene expression product purification and isolation |
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