| 醌还原酶法进行芥蓝抗癌活性的快速检测 |
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| 引用本文： | 张海峰,向珣,王平,汪俏梅.醌还原酶法进行芥蓝抗癌活性的快速检测[J].细胞生物学杂志,2006,28(2):257-260. |
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| 作者姓名： | 张海峰 向珣 王平 汪俏梅 |
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| 作者单位： | 浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室 杭州310029 |
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| 基金项目： | 国家自然科学基金(No.30320974)，人事部留学回国择优资助课题，浙江省自然科学基金(No.R304103)，浙江省科技计划项目(No.2004C32014,No.2005C30012)资助~~ |
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| 摘 要： | 研究了不同提取方法,不同细胞裂解剂和醌还原酶(QR)反应液的培育时间对QR法分析的影响。结果表明,芥蓝组织用磷酸缓冲液(5mmol/LK2HPO4-KH2PO4,1mmol/LEDTA,pH7.6)提取效果良好,与常用的三元试剂提取法效果相当。这种方法简单而有效,优于其他有机溶剂提取法。0.4%乙基苯基聚乙二醇(NP40)可以替代细胞裂解剂——0.8%毛地黄皂苷用于QR分析,且效果良好,QR反应中反应液的培育时间以5￣10min为宜。
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| 关 键 词： | 醌还原酶法 芥蓝 抗癌活性 |
| 收稿时间： | 2005-07-21 |
| 修稿时间： | 2005-09-15 |
Rapid Detection of Anticancer Activity of Chinese Kale by Quinone Reductase Bioassay |
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| Hai-Feng Zhang,Xun Xiang,Ping Wang,Qiao-Mei Wang.Rapid Detection of Anticancer Activity of Chinese Kale by Quinone Reductase Bioassay[J].Chinese Journal of Cell Biology,2006,28(2):257-260. |
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| Authors: | Hai-Feng Zhang Xun Xiang Ping Wang Qiao-Mei Wang |
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| Abstract: | Effect of different extract methods, lysing solutions, and the incubation time of quinonereductase (QR) reaction mixtures on bioassay was studied, and the results showed that the QR inducer potency ofChinese kale phosphate buffer (5 mmol/L K2HPO4-KH2PO4, 1 mmol/L EDTA, pH 7.6) extracts was comparable tothe potency of common triple solvent extracts. Extraction with phosphate buffer was better than other methodsextraction with organic solvents because of its simplicity and effectiveness. 0.4% Nonidet P-40 could be substitutedfor 0.8% digitonin during cell lysis in QR assay. The suitable incubation time of QR reaction mixtures in QR assaywas 5 to 10 minutes. |
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| Keywords: | quinone reductase bioassay Chinese kale anticancer activity |
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