Saturation-transfer difference NMR studies for the epitope mapping of a carbohydrate-mimetic peptide recognized by an anti-carbohydrate antibody |
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| Authors: | Johnson Margaret A Pinto B Mario |
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| Institution: | Departments of Chemistry and of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6. |
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| Abstract: | Saturation-transfer difference NMR spectroscopy (STD-NMR) experiments have been performed to analyze the topography or epitope of the octapeptide MDWNMHAA recognized by the anti-carbohydrate antibody SYA/J6 in solution; the antibody is directed against the Shigella flexneri Y O-antigen polysaccharide. The results permit a valuable comparison of solution versus crystal-structure data, and provide insight for the design of the next-generation binding ligands. |
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