Influence of chloramphenicol on growth and respiration of soybean (Glycine max L.) suspension cultures |
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Authors: | Yvonne M. de Klerk-Kiebert Tarcies J. A. Kneppers Linus H. W. van der Plas |
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Affiliation: | Dept. of Plant. Physiol., Vrije Univ., De Boelelaan 1087, 1081 HB Amsterdam, The Netherlands. |
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Abstract: | By addition of chloramphenicol (CAP) to the growth medium of green soybean ( Glycine max L.) cells in batch culture, growth is inhibited and the activity of the cytochrome oxidase decreases to 60% of the value found in control cells. The presence of CAP induces an enhancement of the contribution of the alternative pathway to total respiration. This enlarged contribution results both from a higher capacity of the alternative pathway and from a greater part of this capacity being used. Also in mitochondria isolated from cells treated with CAP, a higher capacity of the alternative pathway has been found, while the part of this capacity which is really used is comparable with the values found in control cells. |
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Keywords: | Alternative oxidase cell suspension cytochrome oxidase |
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