| 温度对青蒿毛状根生长和青蒿素生物合成的影响 |
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| 引用本文： | 郭晨 刘春朝 叶和春 李国风. 温度对青蒿毛状根生长和青蒿素生物合成的影响[J]. 西北植物学报, 2004, 24(10): 1828-1831 |
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| 作者姓名： | 郭晨 刘春朝 叶和春 李国风 |
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| 作者单位： | 1. 中国科学院过程工程研究所,国家生化工程重点实验室,北京,100080
2. 中国科学院植物研究所,北京,100093 |
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| 摘 要： | 本实验研究了不同温度(15℃～35℃)对青蒿毛状根生长和青蒿素生物合成的影响，发现25℃有利于毛状根生长，30℃促进了青蒿素生物合成。通过温度改变的二步培养技术(培养前20d温度控制在25℃，后10d温度提高到30℃)，青蒿素的产量得到明显提高，高于在恒温培养时(25℃或30℃)的结果。
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| 关 键 词： | 青蒿 青蒿素 毛状根 温度 |
Effect of temperature on growth and artemisinin biosynthesis in hairy root cultures of Artemisia annua |
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| Abstract. Effect of temperature on growth and artemisinin biosynthesis in hairy root cultures of Artemisia annua[J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(10): 1828-1831 |
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| Authors: | Abstract |
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| Abstract: | Temperature effect on growth and artemisinin biosynthesis in the cultured Artemisia annua L. hairy roots was investigated at a range of 15°C ～ 35°C. Maximum hairy root growth was found at 25°C, however, the highest artemisinin content in the root cultures was observed at 30°C. Based on these results, a step-wise temperature shift strategy was developed in which 25°C was used for cultivation for the first 20 days and 30°C for the remainder of the cultivation period. The artemisinin production obtained by the strategy was higher than those in single temperature-control experiments. |
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| Keywords: | Artemisia annua L. artemisinin hairy root cultures temperature |
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