A bicistronic transgene system for genetic modification of <Emphasis Type="Italic">Parthenium argentatum</Emphasis> |
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Authors: | Grisel Ponciano Niu Dong Grace Chen Colleen McMahan |
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Institution: | 1.United States Department of Agriculture,Agricultural Research Service,Albany,USA |
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Abstract: | Parthenium argentatum (guayule) was transformed with a bicistronic transgene containing a viral 2A cleavage sequence. The transgene includes the coding sequences of two key enzymes of the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and farnesyl pyrophosphate synthase (FPPS), involved in rubber biosynthesis. The viral 2A peptide sequence located between the two transgenes allowed for their co-expression via the Arabidopsis CBF2 (C-Binding repeat Factor 2) cold-inducible promoter. We identified three independent transgenic lines expressing the bicistronic transgenes upon cold treatment and examined the rubber content in the in vitro guayule plants. |
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