Histochemical localization of hormone sensitive adenylate cyclase in defined nephron epithelia in culture |
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Authors: | P. D. Wilson M. F. Horster |
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Affiliation: | (1) Department of Medicine, University of Colorado Health Sciences Center, 4200 East 9th Avenue, C281, 80262 Denver, CO, USA;(2) Physiologisches Institut der Universität München, Pettenkoferstrasse 12, D-8000 München 2, Federal Republic of Germany |
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Abstract: | Summary Distal nephron epithelia of defined anatomical origin were microdissected from rabbit kidneys and individually explanted into an in vitro culture system. The 7 day monolayers grown from four different nephron epithelia were studied for the presence and amount of adenylate cyclase reaction product. In each case basal adenylate cyclase was compared with the enzyme reaction product after stimulation by arginine vasopressin, calcitonin, parathyroid hormone (PTH) and isoproterenol. In cortical collecting tubule cultures, the reaction was stimulated by vasopressin >isoproterenol>calcitonin. PTH had no effect. In cortical thick ascending loop of Henle cells, the stimulation was by calcitonin>vasopressin=PTH. Isoproterenol had no effect. In medullary ascending loop epithelia, stimulation was by vasopressin=calcitonin. Neither isoproterenol nor PTH had an effect.These observations indicate that adenylate cyclase is histochemically demonstrable in cultivated cells from rabbit distal nephron segments and that the enzyme activation by hormones is differential according to the epithelium of origin. |
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