Inhibition of growth of Sclerotinia minor and other pathogens by citrinin in the filtrate of Penicillium citrinum

Penicillium citrinum Thom, isolated from the sclerotia of Sderotinia minor, was cultured in a broth of Czapek-Dox for 4 to 8 weeks. The filtrate obtained was incorporated into potato dextrose agar or Czapek-Dox agar at different concentrations (v/v). The amended media were tested for mycelial growth of S. minor and other pathogens. Mycelial growth of S. minor was completely inhibited on media amended with 20% (v/v) filtrate of P. citrinum, and considerable inhibition of S. minor occurred at 10 and 15% concentrations. Mycelial growth of S. major, Sclerotium rolfsii, Rhizoctonia solani (AG-4) was inhibited by similar concentrations of filtrate of P. citrinum. Inhibitor(s) in the filtrate were extracted with ethyl acetate and tentatively identified as citrinin. Citrinin was shown to be an active component in the filtrate against mycelial growth of S. minor, S. major and Sclerotium rolfsii.Cooperative investigation of U.S. Department of Agriculture, Agriculture Research Service and Oklahoma State University. Journal Article No. 4989, Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater.Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by USDA or by Oklahoma State University, or imply their approval to the exclusion of other products or vendors that may also be suitable.