Functional Relationship between Na/K-ATPase and NMDA-Receptors in Rat Cerebellum Granule Cells |
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Authors: | A Boldyrev E Bulygina O Gerassimova L Lyapina W Schoner |
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Institution: | Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia. aaboldyrev@mail.ru |
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Abstract: | Activation of rat cerebellum granule cells by N-methyl-D-aspartate (NMDA, 10(-4)-10(-3) M) results in progressive increase in reactive oxygen species (ROS) and suppression of the ouabain-sensitive part of Na/K-ATPase activity. When Na/K-ATPase was inhibited by high ouabain concentrations (10(-5)-5 x 10(-4) M), an increase in stationary ROS level in neuronal cells was noted, this effect being attenuated by NMDA antagonists, MK-801 and D-AP5. It is concluded that in cerebellum neurons, ouabain-resistant Na/K-ATPase is responsible for suppression of intracellular level of ROS, which, in turn, inhibit ouabain-sensitive Na/K-ATPase. |
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Keywords: | reactive oxygen species (ROS) Na/K-ATPase glutamate receptors NMDA |
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