Cellulase production by Trichoderma reesei (RUT-C30) in fed-batch culture |
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Authors: | T G Watson I Nelligan L Lessing |
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Institution: | (1) Fermentation Technology Division, National Food Research Institute, Council for Scientific and Industrial Research, P.O. Box 395, 0001 Pretoria, Republic of South Africa |
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Abstract: | Summary Fed-batch cultures of Trichoderma reesei RUT-C30 attained quasi-steady state conditions, in respect of biomass concentration and enzyme production rate, commensurate with a specific cell maintenance coefficient of 0.029 g cellulose.g biomass.–1h–1 and specific cellulase production rate of between 9.6 and 11.9 IU (filter paper activity).g biomass.–1h–1. A maximum enzyme yield of 57 IU.m1–1 at an overall productivity of 201 IU.L.–1h–1 resulted from a cellulose feed rate of 1.0g.L.–1h–1. |
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