人单核细胞系U_（937）和T细胞系HSB_2细胞的HSV－1感染

用ＨＳＶ－１接种人单核细胞传代株Ｕ＿（９３７）和Ｔ淋巴细胞传代株ＨＳＢ＿２细胞，通过对病毒滴度、细胞增殖与病变、病毒抗原表达及病毒ＤＮＡ的动态观察，研究了ＨＳＶ－１感染两种细胞的特点。结果发现接种病毒后，Ｕ（９３７）细胞仅允许病毒短时间低滴度复制，培养上清中病毒滴度在１２天内降至测不出水平，细胞经过１～２周后增殖受抑、死亡增多，然后又逐渐恢复；用ＬＰＳ持续刺激则能提高病毒感染滴度，延长感染时间，形成短期持续感染。ＨＳＢ＿２细胞允许病毒复制至较高滴度，呈现急性杀细胞性感染；用ＰＨＡ持续刺激也不改变细胞感染的特点。

Infection of Human Monocyte-Line U_(937)and T-Lymphocyte-Line HSB_2Cells with HSV-1

ells of human monocyte-line U_(937)and human T-lymphocyte-line HSB_2 inoculated withHSV-1 were studied by observing the dynamics of virus titer,cellular proliferation and cy-topathogenic effect,expression of virus antigen and existing time of virus DNA. The resultsshowed that after inoculation,U_(937) cells allowed the virus to replicate only to a quite low de-gree and only for a quite short period.Twelve days after inoculation the virus was not de-tectable in the supernatans. After proliferation inhibltion and death for about 2 weeks , thecells were gradually recovered;stimulation of U(937)cells with LPS increased the virus infectiv-ity ,prolonged the infection period and promoted the formation of temporary persistent infec-tion.The results also showed that HSB_2 cells permited the virus to replicate to a higher titer,and presented an acute and cytocidal infection; stimulation of HSB_2 cells with PHA did notalter the character of the infection of the cells.