Release of Outer Membrane Vesicles from Bordetella pertussis |
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| Authors: | D. Hozbor M.E. Rodriguez J. Fernández A. Lagares N. Guiso O. Yantorno |
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| Affiliation: | (1) Centro de Investigación y Desarrollo en Fermentaciones Industriales, CONICET Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900-La Plata, Argentina , AR;(2) Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900-La Plata, Argentina , AR;(3) Laboratoire des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France , FR |
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| Abstract: | The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines. Received: 22 October 1998 / Accepted: 17 December 1998 |
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